Sponge cells are transferred to cryogenic tubes, which are put into freezer containers containing isopropyl alcohol that slowly freeze the cells at a rate of 1°C (34°F) per minute until they reach -80°C (-112°F).
Cryogenic Tubes
Image courtesy of Exploring the Blue Economy Biotechnology Potential of Deepwater Habitats.
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Sponge cells are transferred to cryogenic tubes, which are put into freezer containers containing isopropyl alcohol that slowly freeze the cells at a rate of 1°C (34°F) per minute until they reach -80°C (-112°F).

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